We are extending synthetic protein chemistry into the live-cell environment to study protein function in real-time. This involves developing innovative delivery platforms, such as cR10-DABCYL and Suspension Bead Loading (SBL), to transport synthetic proteins across the plasma barrier with high efficiency. These tools enable us to “spy” on intracellular processes, monitoring protein localization, trafficking, and turnover to better understand cellular proteostasis.
Selected References:
- Angew. Chem. Int. Ed. 2024, 63, e202410135. (Suspension Bead Loading (SBL) protein delivery)
- Angew. Chem. Int. Ed. 2022, 21;61(47):e202207551. (Structure-Uptake Relationship Study of DABCYL-Derivatives Delivery)
- Acc. Chem. Res. 2022, 55, 2055. (Synthetic proteins as “molecular spies” behind the plasma barrier)
- Angew. Chem. Int. Ed. 2021, 60, 7333. (Enhanced live-cell delivery via DABCYL fusion)
